Figure 4.

Role of MCP3 NTD in catalysis and homodimerization. (A) 12% SDS-PAGE analysis of 3 μg WT and ΔN MCP3 compared to a 3 μg BSA standard. (B) Deacylation of Ala-tRNA^Pro by WT and ΔN MCP3 at 27°C under multiple-turnover conditions (20 nM protein, 200 nM Ala-tRNA^Pro). (C) Representative ITC results of WT MCP3 homodimer dissociation performed at 25°C as described in methods. Reported K_Dimer represents an average of three trials with standard deviation indicated. (D) SEC-MALS analysis of WT (31 kDa, pink trace) and ΔN MCP3 (23 kDa, teal trace), and tRNA^Pro alone (25 kDa, brown trace). The calculated molar masses represent an average of three independent trials with standard deviation indicated.