Figure 5.

MCP3 NTD plays minor role in tRNA^Pro binding. (A) SEC-MALS analysis of WT (pink trace) and ∆N MCP3 (blue trace) in the presence of tRNA^Pro. The tRNA was pre-incubated with 2.5-fold molar excess of MCP3 at 27°C prior to SEC-MALS analysis at 4°C. Annotated molar masses represent an average of three trials with standard deviation indicated. (B,C) ITC analysis of binding between WT MCP3 (B) or ∆N MCP3 (C) and tRNA^Pro at 25°C. Average K_D values and thermodynamic parameters are summarized in Table 3.