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. 2024 Jul 16;15:1445687. doi: 10.3389/fmicb.2024.1445687

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Copyright © 2024 Watkins, Vradi, Shulgina and Musier-Forsyth.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Acceptor stem specificity of MCP3 and ProRS. (A) Cloverleaf model of Tb tRNA^Pro_(UGG) with mutations indicated: G1C:C72G (red), C73A (blue). (B) Single-turnover deacylation of WT (teal), G1C:C72G (red), C73A (grey), and G1C:C72G, C73A (green) Ala-tRNA^Pro by MCP3 at 13.5°C. (C) Single-turnover deacylation of WT (teal), G1C:C72G (red), C73A (grey), and G1C:C72G, C73A (green) Ala-tRNA^Pro by FL ProRS at 27°C. Error bars represent the average of three independent trials. Rate constants are summarized in Table 4.