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. 2024 Jul 16;15:1445687. doi: 10.3389/fmicb.2024.1445687

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Copyright © 2024 Watkins, Vradi, Shulgina and Musier-Forsyth.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tb MCP3 and ProRS deacylation of G1:C72-containing tRNAs with altered discriminator base. (A) Acceptor stem sequences of Tb tRNAs mis-charged with alanine and tested for deacylation. Colors and symbols match the deacylation curves in panels (B,C). (B) Single-turnover deacylation of Ala-tRNAs by MCP3 at 27°C. Error bars represent an average of three trials. (C) Single-turnover deacylation of Ala-tRNAs by FL ProRS at 27°C. Error bars reflect the standard deviation between three trials. Results of deacylation of Ala-tRNA^Thr, tRNA^Trp, and tRNA^Leu by ProRS are based on a single trial. Rate constants are summarized in Table 5.