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MCP3 deacylation and interaction with Ala-tRNAAla. (A) Deacylation of Ala-tRNAAla (red ■) and Ala-tRNAPro (teal ●) by MCP3 at 13.5°C. Error bars indicate the average of three independent trials. (B) ITC analysis of MCP3 binding to tRNAAla at 25°C. Average KD values and thermodynamic parameters are summarized in Table 3.
