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Single-turnover rate constants for deacylation of Ala-tRNAPro by MCP3, FL ProRS, and the ProXp-ala domain of Tb ProRS.
| Enzyme | kobs (sec−1)* | Fold-Change† |
|---|---|---|
| MCP3 | 0.329 ± 0.055 | – |
| FL ProRS | 0.046 ± 0.010 | 7 |
| ProXp-ala | 0.009 ± 0.001 | 37 |
*kobs values determined using 500 nM enzyme and 50 nM Ala-tRNAPro at 27°C. Standard deviations represent the average of three independent trials. †Fold-change is based on MCP3 activity.
