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Summary of thermodynamic parameters of WT and ΔN MCP3 binding to tRNA measured by ITC.†
| KD (μM) | ΔH (kcal/mol) | −TΔS (kcal/mol) | ΔG (kcal/mol) | |
|---|---|---|---|---|
| WT MCP3: tRNAPro | 1.05 ± 0.348 | −24.3 ± 2.80 | 16.2 ± 2.95 | −8.20 ± 0.283 |
| ΔN MCP3: tRNAPro | 0.809 ± 2.33 | −7.98 ± 4.70 | −3.55 ± 4.50 | −8.33 ± 0.177 |
| WT MCP3: tRNAAla | 1.12 ± 0.300 | −4.70 ± 0.540 | −3.88 ± 0.790 | −8.6 ± 0.309 |
†Values represent the average of three independent trials with standard deviations indicated.
