Table 5.
Single-turnover rate constants for deacylation of various tRNAs mischarged with alanine by MCP3.
| Ala-tRNAi | MCP3 kobs (sec−1)* | FL ProRS kobs (sec−1)⧫ |
|---|---|---|
| Pro | 0.329 ± 0.056 | 0.030 |
| Ala | 0.310 ± 0.080 | 0.002 |
| Leu | 0.180 ± 0.069 | ND |
| Thr | 0.018 ± 0.005 | ND |
| Trp | 0.032 ± 0.007 | ND |
*kobs values determined using 500 nM enzyme and 50 nM Ala-tRNA at 27°C. Standard deviations represent the average of three independent trials. ND, not detected. ⧫kobs reported for FL is a single trial (n = 1) determined using 500 nM enzyme and 50 nM Ala-tRNA at 27°C. ℹAmino acid in first column refers to the identity of the tRNA acceptor.