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Single-turnover rate constants of Ala-tRNAAla protection assays with MCP3.
| Ala-tRNAAla Protection | MCP3 kobs (sec−1)* | Fold-change† |
|---|---|---|
| MCP3 alone | 0.013 ± 0.004 | – |
| MCP3 + FL ProRS | 0.007 ± 0.002 | 1.9 |
| MCP3 + AlaRS | ND | >30 |
| MCP3 + Hs LysRS | 0.019 ± 0.004 | +1.5 |
*kobs values determined using 500 nM enzyme, 500 nM ARS, and 50 nM Ala-tRNAAla at 13.5°C. Standard deviations represent the average of three independent trials. ND, not detected. †Fold-change is based on MCP3 activity.
