Figure 4.

BCG-induced FcγRIV expression was maintained after Th vaccination. Mice (n = 10) were sham vaccinated with PBS or with BCG at 10⁷ CFU per mouse and rested for 17 weeks. Each group was further subdivided into 2 groups (n =5) and received either PBS or the Th GP-FL vaccine at 0.05 μg per mouse in alum, and splenocytes were harvested 2 weeks later to determine the level of FcγRIV expression by FACS. Singlet gating on splenocytes were stained for macrophages (CD11b^high Ly6C^high), neutrophils (CD11b^high Ly6G^high), and NK cells (NK1.1^high) for expression of FcγRIV (CD16.2) as shown in (A) for representative animals of sham vaccinated with PBS and with BCG alone, and quantified in (B) by MFI levels for the 5 animals in each group. The median with interquartile is shown and analyzed using Kruskal-Wallis one way ANOVA nonparametric test with and Dunn’s multiple comparison test; (**p < 0.01 and *p < 0.05).