Figure 5.

Long lasting humoral immune response induced by the Th vaccine. (A) Serum samples from sham PBS (□) or BCG (●) vaccinated mice and subsequently immunized once with 5 μg of the Th GP-FL vaccine were analyzed by ELISA for anti-EBOV GP IgG1 (left panel) or IgG2c (right panel) antibodies throughout the time course of 39 weeks. Serum from a naïve mouse (⨂) collected at week 2 and 4 was used to measure the background level for the ELISA assay. (B) Splenocytes and bone marrow cell suspensions from these mice at 39 weeks were harvested and used for B cell ELISPOT assay to quantitate anti-EBOV GP IgG1 or IgG2c antibody secreting spot forming cells (SFCs). The median values with interquartile ranges are shown and analyzed using Kruskal-Wallis one way ANOVA nonparametric test with Dunn’s multiple comparison test; (*p < 0.05, **p < 0.01, ***p< 0.001).