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. 2024 Jun 24;38(8):1731–1741. doi: 10.1038/s41375-024-02296-6

Fig. 2. PPMX-T003 caused DNA double-strand breaks to S-phase ANKL cells, similar to conventional cytotoxic agents.

Fig. 2

a A schema of in vivo CRISPR screening targeting iron-require molecules. The procedure was independently performed three times, and three mice (Mouse #1, Mouse #2, and Mouse #3) were analyzed. b Negative selection-Robust Ranking Algorithm (RRA) scores of all genes of Mouse #1. The blue dots indicate the RRA value of sgRNAs targeting CIAO1and POLA1, and control (non-target) sgRNA (CTRL). c All annotated gene sets targeted by negatively selected sgRNAs in Mouse #1 using GSEA. Color value and red characters indicate the false-discovery ratio (FDR) and five commonly annotated gene sets in all the three mice (Mouse #1, #2, and #3; see Supplementary Fig. S2c, d). d Flow cytometric analyses of NK92 and KHYG1 exposed to PPMX-T003. Cells were cultured for 24 h with or without 10 µg/mL of PPMX-T003 before analyses. Relationships of cell cycle (DNA content) and DNA damage were plotted. e, f Flow cytometric analyses of liver- and spleen-derived ANKL1 cells of PBS- or PPMX-T003-treated ANKL1-PDXs. Total of three mice per treatment group were analyzed.