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. 2024 Jun 24;38(8):1731–1741. doi: 10.1038/s41375-024-02296-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a A schema of sample preparation for single-cell whole-transcriptome analysis. b Unsupervised clustering (UMAP) of analyzed ANKL1 cells. The bar graph indicates the cell populations of each cluster. c GSEA of feature genes in cluster 1. All annotated gene sets in HALLMARK of MSigDB with adjusted p-values lower than 0.25 were described. MYC (d) and TFRC (e) expression profiles of all the analyzed spleen-derived cells per cluster. Color consistency represents the amount of expression in each cell type. f Results of cell cycle scoring analysis of all the analyzed spleen-derived cells (left) and its schema describing cell cycle transition (right).