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. 2024 Jun 24;38(8):1731–1741. doi: 10.1038/s41375-024-02296-6

Fig. 5. Amino acid influx via LAT1 is essential to the therapeutic efficacy of PPMX-T003 through positive regulation of mTOR/Myc activity.

Fig. 5

Proliferation assay of ANKL-derived cell lines (a), liver-derived ANKL-PDX cells (b), and normal lymphocytes derived from peripheral blood of healthy volunteer (b) treated with various concentrations of JPH-203 for 48 h (a) or 24 h (b). Total of three samples per treatment group were analyzed. c Cell cycle assays of JPH-203-treated NK92 and KHYG1 cells. After cultivation with 40 µM JPH-203 for 24 h, cell cycle proportions were measured using flow cytometry. Total of three samples per treatment group were analyzed. d Western blot analysis of JPH-203-treated NK92 and KHYG1 cells. After cultivation with 40 µM of JPH-203 for 0, 6, or 24 h, the relative luminescent intensities of each band compared with those of β-actin were calculated and described under the band. e Cellular γH2AX expression value of NK92 and KHYG1 cells cultured with or without 40 µM of JPH-203 and/or 10 µg/mL of PPMX-T003 for 24 h, measured using flow cytometry. Total of three samples per treatment group were analyzed. f In vivo luciferase assay for ANKL3-PDXs treated with PPMX-T003 and DMSO or JPH-203 sequentially.