Fig. 1.

UBXN3B controls B cell development in a B cell-intrinsic manner. a, b) Irradiated wild type (WT, CD45.1) recipient mice were transplanted with ERT2-Cre⁺Ubxn3b^flox/flox bone marrow (CD45.2). The mice were then treated with tamoxifen (TMX) to delete Ubxn3b in haematopoietic cells (designated Ubxn3b^−/− BM–WT) or corn oil (designated Ubxn3b^+/+ BM–WT). c, d) Irradiated Cre⁺Ubxn3b^flox/flox and Ubxn3b^flox/flox (CD45.2) mice were transplanted with wild type (WT, CD45.1) bone marrow. The mice were then treated with TMX to delete Ubxn3b (designated WT BM–Ubxn3b^−/−) or not (designated WT BM–Ubxn3b^+/+) in non-haematopoietic cells. Data shown in a-d) are the cellularity of major blood immune populations at 45 days after TMX. e) The cellularity of blood immune cells in Mb1-Cre^−/−Ubxn3B^WT/WT (Mb1^WTUbxn3b^WT), Mb1-Cre^−/+Ubxn3B^WT/WT (Mb1^HetUbxn3b^WT), Mb1-Cre^−/+Ubxn3B^flox/WT (Mb1^HetUbxn3b^Het), Mb1-Cre^+/+Ubxn3B^WT/WT (Mb1^KOUbxn3b^WT), and Mb1-Cre^−/+Ubxn3B^flox/flox (Mb1^HetUbxn3b^KO) mice. RBC: red blood cells, WBC: white blood cells. The horizontal line indicates the median of the result. Each symbol = one mouse. In a, b) N = 5, c, d) N = 4, e) N = 3 mice/genotype. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 [multiple unpaired Mann–Whitney tests with Holm-Šídák correction for a; multiple unpaired Welch t-tests with Holm-Šídák correction for b-d; two-way ANOVA with two-stage step-up method of Benjamini, Krieger and Yekutieli multiple comparisons test after log-transformation for e].