Table 2. Scoring Rubric for the ENI as Based on the Dietary Reference Intakes^a.

Nutrient	Criteria for 0 points (minimum score)	Criteria for 1 point	Criteria for 2 points (maximum score)
Vitamin A (total activity in retinol equivalents), μg/db	<EAR (500)	EAR ≤ x < RDA	≥RDA (700)
Vitamin C (total ascorbic acid), mg/d	<EAR (60)	EAR ≤ x < RDA	≥RDA (75)
Vitamin E (total α-tocopherol), mg/d	<EAR (12)	EAR ≤ x < RDA	≥RDA (15)
Folate (total folate), μg/dc	<EAR (320)	EAR ≤ x < RDA	≥RDA (400)
Vitamin B12 (total cobalamin), μg/d	<EAR (2)	EAR ≤ x < RDA	≥RDA (2.4)
Zinc, mg/d	<EAR (6.8)	EAR ≤ x < RDA	≥RDA (8)
Selenium, μg/d	<EAR (45)	EAR ≤ x < RDA	≥RDA (55)
Magnesium, mg/d	<EAR (265)	EAR ≤ x < RDA	≥RDA (320)
Dietary fiber (total dietary fiber), g/d	<AI/1.2	AI/1.2 ≤ x < AI (25)	≥AI (25)
MUFA:SFA (as calories from MUFA:calories from SFA)	<Medianas	Medianas ≤ x < 1.2 × medianas	≥1.2 × Medianas
Isoflavones (sum of daidzein, genistein, glycitein, biochanin A, and formononetin), mg/d	<Medianas	Medianas ≤ x < 1.2 × medianas	≥1.2 × Medianas
Total sugar, g/d	≥1.2 × 90 g	90 < x < 1.2 × 90	≤90
Sum each component’s score to obtain the total ENI score	0	NA	24

Abbreviations: AI, adequate intake; EAR, estimated average requirement; ENI, Epigenetic Nutrient Index; median_as, median of the analytic sample; MUFA, monounsaturated fatty acids; NA, not applicable; RDA, recommended dietary allowance; SFA, saturated fatty acids.

^a Values corresponding to the EAR, RDA, and AI for each nutrient component are noted (in parentheses) as based on age- and sex-based intake estimates put forth by the Food and Nutrition Board of the US Institute of Medicine and the National Health Service of the United Kingdom (here, values correspond to those for women aged 36-43 years).³⁴

^b Not accounting for differences in vitamin A activity of carotenoids.³⁵

^c Not accounting for differences in folate absorption between natural and synthetic forms.³⁵