Figure 2 -.
Nitrite release from DC 2.4 cells was measured by Griess assay. Murine DC 2.4 cells were pulsed with LPS (5 μg) as positive control, pDNA (1 μg), blank NP (50 μg), pDNA nanoparticulate vaccine (50 μg, pDNA amount 1 μg), and pDNA nanoparticulate vaccine plus adjuvants Alum and MF59. There was a significant increase in nitrite release by groups received pDNA nanoparticulate vaccine with or without adjuvant Alum and MF59 (*p<0.05 significant, **p<0.01very significant, ***p<0.001 extremely significant) measured using one-way ANOVA with Tukey post hoc test. Data are expressed as mean ± SEM.