Figure 4 -.
Antibody titers (IgG) in mice after rabies vaccination administered via intramuscular route (IM). Mice (n=6) were vaccinated on day 1 with pDNA (100 μg) in hydrogel, pDNA nanoparticulate vaccine in hydrogel, and pDNA nanoparticulate vaccine plus adjuvant in hydrogel. Blood was collected every week post-vaccination and analyzed for anti-GnRH antibodies using ELISA. Results were compared statistically among groups every week (*p<0.05 significant, **p<0.01very significant, ***p<0.001 extremely significant) using one-way ANOVA with Tukey post hoc test. Data are expressed as mean±SEM.