TABLE 3.
PCR results for 5,000-neuron samples from eight subjects
| Subject no./sidea | PCR results for:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Mixed cellsb
|
Neuronal cellsb
|
Nonneuronal cellsb
|
|||||||
| Actin | ORF21/Q | ORF21/N | Actin | ORF21/Q | ORF21/N | Actin | ORF21/Q | ORF21/N | |
| 1/L | + | 4 | + | + | 0 | + | + | 0 | − |
| 1/R | + | 0 | − | + | 1 | + | + | 0 | − |
| 2/L | + | 0 | − | + | 0 | − | + | 0 | − |
| 2/R | + | 3 | + | + | 2 | + | + | 1 | + |
| 3/L | + | 0 | − | + | 0 | − | + | 0 | − |
| 3/R | + | 0 | − | + | 0 | − | + | 0 | − |
| 4/L | + | 0 | + | + | 1 | + | + | 0 | − |
| 4/R | + | 0 | − | + | 1 | + | + | 0 | − |
| 5/L | + | 2 | + | + | 1 | + | + | 0 | − |
| 5/R | + | 3 | + | + | 12 | + | + | 0 | + |
| 6/L | + | 0 | − | + | 0 | − | + | 0 | − |
| 6/R | + | 0 | − | + | 0 | − | + | 0 | − |
| 7/L | + | 0 | − | + | 0 | − | + | 0 | − |
| 7/R | + | 0 | − | + | 0 | − | + | 0 | − |
| 8/L | + | 4 | + | + | 2 | + | + | 1 | + |
| 8/R | + | 0 | + | + | 0 | + | + | 0 | + |
a
L, left TG; R, right TG.
b
Actin, primary PCR (40 cycles). The presence (+) or absence (−) of the β-actin gene in the sample is indicated. ORF 21Q, primary PCR (40 cycles), followed by nested PCR (10 cycles). The number of VZV gene 21 copies after quantitation against standards is indicated. ORF 21N, primary PCR (40 cycles), followed by nested PCR (40 cycles). The presence (+) or absence (−) of VZV gene 21 at the highest possible sensitivity is indicated.