(A) Generation of MUFA products by cellular SCD1 activity; SCD1 products are in red, and elongation products are in gray. (B-C) Scd1 expression in chow-fed or fasted and sucrose-refed animals in proximal, middle, and distal small intestine and colon; n=5–8. Averages ± SEM, bars with different letters are significantly different from each other. (D,E) SCD1 protein expression in the proximal, middle, and distal intestine and colon of chow-fed and sucrose-fed mice. Each lane contains protein pooled from 3 animals; *, p<0.05 vs. proximal (F) Immunohistochemical staining for SCD1 in intestines; images representative of three animals each; scale bar represents 25 μM. SCD1, Stearoyl-CoA Desaturase 1, β-Actin, Beta-Actin, GAPDH, glyceraldehyde 3-phosphate dehydrogenase.