(A) Model for the generation of an intestinal SCD1 knockout model. (B, C) Intestinal SCD1 gene and protein expression is significantly reduced in small intestine and colon of chow fed mice. (D,E) SCD1 gene and protein expression is not changed in non-intestinal tissues. (F-H) Body weights and body composition are not genotypically different in chow-fed mice. Averages ± SEM. *p<0.05 vs fl/fl. n= 6–8 for gene expression and body composition, and protein pooled from 3 animals per lane for protein blots. SCD1, Stearoyl-CoA Desaturase 1, GAPDH, glyceraldehyde 3-phosphate dehydrogenase.