FIG. 4.

Binding of MLV vector particles to TE671 cells. Cell supernatant of TE671 cells producing LacZ(MLV-A) (A), LacZ(MLV-E) (B), LacZ pseudotype without Env proteins (C), and no virus particle (D) was added to TE671 cells. The cells were washed extensively after a 1-h incubation and then fixed with acetone-methanol (1:1). Samples were stained for MLV CA proteins with FITC-labeled secondary antibodies. Bar, 5 μm.