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. 2024 Jul 29;22:702. doi: 10.1186/s12967-024-05495-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Function analysis of NUhighepi/NUlowepi in scRNA-seq data: (A) Barplot showed the number of other cell types communicating with NU group. (B-C) Pseudotime analysis of epithelial cells. (D) Heatmap showed pseudotime of Nucleotide metabolism related genes (NRGs). (E-F) Barplot showed pseudotime states (E) or cell-cycle states (F) of NU group. (G) Heatmap showed inferCNV profiles of NUhighepi and NUlowepi. (H) Heatmap showing transcriptional activity of NU group. Intensity of color indicates average transcription factors activity. (I) Raincloud plot of CytoTRACE scores by NU group in scRNA-seq data. The center of the box plot are median values, the bounds of the box are 25% and 75% quantiles. (J) Scatter plot of the correlation between CytoTRACE score and Nucleotide metabolism score. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, ns P > 0.05