Fig. 6.

Formation of ALDH2 dimers and tetramers. (A) Representative western blot of wild type ALDH2 and the ALDH2 variants using a S-tag antibody with quantification of dimer and tetramer formation detected by the S-tag antibody. The R101G variant did not form dimers and tetramers. (B) Representative western blot of wild type ALDH2 and the ALDH2 variants using an ALDH2 antibody with quantification of dimer and tetramer formation by the ALDH2 antibody. MW = molecular weight markers, n = 5 or 6/group, ^*p < 0.01 by one-way ANOVA relative to wild type, R114W and E504K