Table 1.
Analysis of marine mammal placentas for Coxiella burnettii infection.a
| Identifier | Species | Date | Status of fetus | IS1111b | Com1b | IHCc | Placentitisd | Serume |
|---|---|---|---|---|---|---|---|---|
| GI06-04 | Harbor seal | 3 Jun 2006 | 3rd trimester premature pup | 33 | 35.8 | neg | No | n.t. |
| SMI P No. 1 | Harbor seal | 12 Jul 2006 | Pup status unknown | 35.3 | undet. | neg | No | n.t. |
| SMI P No. 2 | Harbor seal | 12 Jul 2006 | Pup status unknown | 37 | undet. | neg | No | n.t. |
| SMI P No. 3 | Harbor seal | 12 Jul 2006 | Pup status unknown | 39 | undet. | n.t. | No | n.t. |
| SMI P No. 4 | Harbor seal | 12 Jul 2006 | Pup status unknown | 38.5 | undet. | neg | No | n.t. |
| SMI P No. 5 | Harbor seal | 12 Jul 2006 | Pup status unknown | undet. | undet. | neg | No | n.t. |
| SMI P No. 7 | Harbor seal | 12 Jul 2006 | Pup status unknown | 37 | undet. | pos | Yes | n.t. |
| GI P 2006 No. 1 | Harbor seal | 24 Jul 2006 | Pup status unknown | undet. | undet. | neg | No | n.t. |
| GI P 2006 No. 2 | Harbor seal | 24 Jul 2006 | Pup status unknown | 38 | undet. | neg | No | n.t. |
| GI P 06/3286 | Harbor seal | 1 Aug 2006 | Live birth, full term pup | 33.6 | 35.8 | neg | Yes | n.t. |
| WDFW0906-01 | Harbor seal | 7 Sep 2006 | Full term, stillborn | 38 | 38.8 | neg | No | n.t. |
| WDFW1106-03 | Harbor seal | 11 Nov 2006 | 2nd trimester, in mother | undet. | undet. | neg | No | pos |
| WDFW0207-01 | Harbor seal | 9 Feb 2007 | 2nd trimester, aborted | 35 | undet. | neg | No | n.t. |
| WDFW0607-01 | Harbor seal | 1 Jun 2007 | 3rd trimester, in mother | undet. | undet. | neg | No | n.t. |
| WB 2007 No. 3 | Harbor seal | 18 Jul 2007 | Pup status unknown | 36.1 | 38.6 | n.t. | n.t. | n.t. |
| WDFW2008-048 | Harbor seal | 21 May 2008 | 3rd trimester, in mother | undet. | undet. | n.t. | n.t. | n.t. |
| WDFW2008-053 | Harbor seal | 28 May 2008 | Near term, in mother | 36.3 | 32.4 | pos | Yes | pos |
| GI P 2008 No. 1 | Harbor seal | 1 Jul 2008 | Pup status unknown | 34.1 | undet. | n.t. | n.t. | n.t. |
| GI P 2008 No. 2 | Harbor seal | 15 Jul 2008 | Pup status unknown | 33.4 | 36.8 | n.t. | n.t. | n.t. |
| 09Pv11MayWI-10 | Harbor seal | 11 May 2009 | 3rd trimester, in mother | undet. | undet. | n.t. | n.t. | n.t. |
| CRC-P A | Harbor seal | 9 Jul 2009 | Pup status unknown | undet. | undet. | neg | No | n.t. |
| CRC-P B | Harbor seal | 9 Jul 2009 | Pup status unknown | 37.4 | undet. | neg | No | n.t. |
| GI09-35 | Harbor seal | 31 Jul 2009 | Full term, stillborn | 34.3 | 38 | neg | Yes | n.t. |
| CRC-963 | Harbor seal | 15 Aug 2009 | Full term, stillborn | 35.1 | 39 | neg | No | n.t. |
| WDFW2010-079 | Harbor seal | 18 Jun 2010 | Live, premature pup | undet. | undet. | n.t. | n.t. | n.t. |
| CRC-1055 | Harbor seal | 29 Jun 2010 | Full term, stillborn | undet. | undet. | n.t. | n.t. | n.t. |
| Plac-WB-072310 | Harbor seal | 26 Jul 2010 | Pup status unknown | undet. | undet. | n.t. | n.t. | n.t. |
| CRC-798 | Harbor porpoise | 11 Jul 2007 | Near term, in mother | 35 | 39.9 | n.t. | No | n.t. |
| CRC-835 | Harbor porpoise | 14 Mar 2008 | 3rd trimester, in mother | undet. | undet. | n.t. | No | n.t. |
| CRC-922 | Harbor porpoise | 13 Apr 2009 | 3rd trimester, in mother | 35.7 | undet. | n.t. | No | n.t. |
| CRC-1063 | Harbor porpoise | 9 Jul 2010 | Near term, in mother | undet. | undet. | n.t. | No | neg |
| WDFW2010-170 | Harbor porpoise | 15 Oct 2010 | 1st trimester, in mother | undet. | undet. | n.t. | No | pos |
| 10Pp31DecWI-07 | Harbor porpoise | 31 Dec 2010 | 2nd trimester, in mother | undet. | undet. | n.t. | n.t. | n.t. |
| WDFW0307-03 | Steller sea lion | 27 Mar 2007 | 2nd trimester, in mother | 37 | 38.5 | n.t. | Yes | n.t. |
| WDFW2010-200 | Steller sea lion | 20 Nov 2010 | 2nd trimester, in mother | undet. | undet. | n.t. | No | n.t. |
Harbor seal (Phoca vitulina richardsi), harbor porpoise (Phocoena phocoena), and Steller sea lion (Eumetopias jubatus) placentas were collected in the Pacific Northwest, USA.
The threshold cycle for quantitative polymerase chain reaction (PCR) targeting IS1111a or com1; undet. = undetermined; samples for which fluorescence did not cross the threshold after 40 cycles of PCR.
pos = sample stained with anti–C. burnetii murine hyperimmune ascites fluid; n.t. = not tested; neg = no staining detected.
Yes = sample showed evidence of placentitis based on H and E staining; no = no evidence of placentitis; n.t. = not tested.
pos = serum sample was positive in indirect fluorescent antibody test using anti-dog secondary reagent; n.t. = not tested; neg = negative indirect fluorescent antibody test.