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. 2024 Jul 18;20(7):e1012411. doi: 10.1371/journal.ppat.1012411

Fig 7. BCC0 residues 570–899 bind to IMC32’s C-terminal coiled-coil domains.

Fig 7

A) Diagram of the fragments of BCC0, IMC32, and IMC43 used for pairwise Y2H assays. Residues included in each fragment are indicated. “Myr.” = predicted myristoylation site. “Palm.” = predicted palmitoylation site. “CC” = coiled-coil domain. “LOC” = IMC43 localization domain. “EID” = IMC43 essential interaction domain. B) Pairwise Y2H assays testing for binding between fragments of BCC0 and IMC32. Pairs of fragments that were unable to be tested due to auto-activation of both fragments in the pB27 vector are indicated. Growth on permissive (-L/W) media indicates the presence of both the pB27 and pP6 bait and prey plasmids. Growth on restrictive (-L/W/H) media indicates binding between the indicated fragments of each protein. C) Pairwise Y2H assays testing for binding between fragments of BCC0 and IMC43.