FIG. 9.

Productive viral gene expression occurs in PBMC and lymphoid tissue. RNA extraction, RT, and PCR were performed as described in Materials and Methods. cDNAs were PCR amplified with bICP0 (A), RR (B), and gC (C) primers. Lanes: 1 and 16, molecular weight marker (100-bp DNA ladder); 2 and 3, MDBK cells infected with BHV-1 without and with RT, respectively; 4 and 5, PBMC from a mock-infected calf without and with RT, respectively; 6 and 7, CD4⁺ T cells prepared from a mock-infected calf without and with RT, respectively; 8 and 9, CD8⁺ T cells from a mock-infected calf without and with RT, respectively; 10 and 11, PBMC from a BHV-1-infected calf without and with RT, respectively; 12 and 13, CD4⁺ T cells from a BHV-1-infected calf without and with RT, respectively; 14 and 15, CD8⁺ T cells from a BHV-1-infected calf without and with RT, respectively.