TABLE 1.

Flow cytometric analysis of CD4⁺ and CD8⁺ T lymphocytes from PBMC^a

T lymphocytes	% of total T lymphocytes (no. of calves tested)
	Mock	5 dpi	7 dpi	9 dpi	12 dpi	16 dpi
CD4+	20.5 (3)	15.5 (4)b	14.5 (4)b	15.1 (3)b	15.8 (4)b	18.8 (2)
CD8+	13.6 (3)	13.4 (4)	9.4 (4)b	9.1 (3)b	15.2 (4)	14.7 (2)

^aCalves that were seronegative for BHV-1 were inoculated with 10⁷ 50% tissue culture infective doses of BHV-1 per ml. At the indicated times, CD4⁺ and CD8⁺ T lymphocytes were prepared from PBMC after density gradient centrifugation on Histopaque 1083. Mononuclear cells were incubated with a monoclonal antibody directed against bovine CD4 or anti-bovine CD8. A secondary antibody conjugated to FITC or PE was then incubated with the samples, and flow cytometry was performed. 

^bSignificant difference (P < 0.05, independent t test) compared with mock-infected calves.