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. Author manuscript; available in PMC: 2024 Jul 30.
Published in final edited form as: FASEB J. 2022 Jun;36(6):e22335. doi: 10.1096/fj.202101518R

FIGURE 4. SIRT6 blunts stress-induced expression of SREBP target genes in SPTBN1+/− MEFs.

FIGURE 4.

MEFs were transfected with empty vector (EV) or FLAG-SIRT6 and exposed to vehicle (Veh) or stressed with TNF-α (20 ng/ml) and cycloheximide (CHX, 10 μg/ml) for 3 h. (A) In addition to the EV or FLAG-SIRT6, MEFs were transfected with plasmids encoding Renilla and luciferase controlled by the indicated promoter. Luciferase activity from the LDLR promoter or the SCD1 promoter was normalized using the activity of Renilla luciferase and the mean ± SEM relative to the vehicle-treated WT were plotted (n = 3 – 4 independent experiments). (B) Transcript abundance of the indicated SREBP target genes is presented relative to that in WT MEFs transfected with empty vector and exposed to vehicle. Data are presented as the mean ± SEM (n = 3). In A and B, *, p < 0.05, compared with vehicle-treated cells; #, p < 0.05, stressed SPTBN1+/− EV versus FLAG-SIRT6.