Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Examples of plasmids for bacterial expression of LASL components
| Construct insert | Promoter | Replication | Purification Tag | Vector, resistance | Linker |
|---|---|---|---|---|---|
| nPOI–linker–ST–6×His | T7 | P15A | 6×His | pET-29a (kanamycin) | GGSGGGGSGGS |
| SC–linker–cPOI–6×His or SC–linker–cPOI–LPETG–6×His | T7 | P15A | 6×His | pET-21a (carbenicillin) | GGSGGGSGG |
| (MmPylRS; Y306M, L309A, C348A, Y384F) x2–Pyl–tRNACUA | araBAD, glnS’ | P15A | – | pEVOL (chloramphenicol) | – |
