Table 3 |.

Examples of plasmids for mammalian expression of LASL components

Construct insert examples	Promoter	Vector, resistance	Linker, P2A AA sequence
SC–linker1–cPOI–P2A–nPOI–linker2–ST–P2A–mCh	CMV	pcDNA3.1 (carbenicillin, HygR)	Linker 1: GGSGGGSGG Linker 2: GGSGGGSGGGSG P2A: GSGATNFSLLKQAGDVEENPGP
nPOI–ST–P2A–SC–cPOI	CMV	pcDNA3.1 (carbenicillin, HygR)
POI–linker1–ST–P2A–SC–Iinker2–CAAX	CMV	pcDNA3.1 (carbenicillin, HygR)	Linker 1: GGSGGGSGGGSG Linker 2: GGSGGGSGG P2A: GSGATNFSLLKQAGDVEENPGP CAAX: MSKDGKKKKKKSKTKCVIM
hMbPylRS(Y271A, Y349F)–4x(M15-tRNACUA)	CMV, U6	pcDNA3.1 (carbenicillin, NeoR)	—