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. 2024 May 9;195(4):2985–2996. doi: 10.1093/plphys/kiae230

Figure 6.

Figure 6.

NBlab07G09180, encoding a bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 (NEC3), mediates cell death induction in N. benthamiana. A) Graphical representation of the NEC3 promoter region. Gene-specific dTALEs (dTalG2.1 to dTalG2.6) are underlined. dTal26/dTal50/dTal55 effector-binding element is highlighted in gray. Arrow indicates TSS. The start codon (ATG) is shown in bold italics. B) Cell death response on leaves infiltrated via Agrobacterium with 35S::NEC3 and gene-specific dTALEs. Pictures were taken at 6 dpi.C)NEC3 mRNA levels analyzed by RT-qPCR at 48 hpi for dTALEs and 72 hpi for 35S::NEC3. Results are presented as mean ± SD. * significant after t test (P-value < 0.005). Control: infiltration medium. Three replicates per sample were performed. CD, cell death.