Table 1.

Overview of MeSWEET10a mutant line genotypes

Mutant type	General description	Number of lines
WT-like transgenic	Transgenic lines with no edits at gRNA target sites	1
Silent mutant	Edits that do not impact coding sequence	13
TAL20 EBE promoter INDEL	Edits within the TAL20 EBE with intact TATA box	1a
MeSWEET10a promoter INDEL	Edits in the TATA box predicted to impact expression	11
MeSWEET10a frameshift	Edits after the TSS expected to impact coding sequence	3

Mutation type summary of the 29 transgenic lines recovered from all rounds of transformation based on Sanger sequencing results.

^aA line that died during the tissue culture process.