FIG. 4.

Binding of DHBV virions to several DCPD-reconstituted cell lines. (A) Transiently transfected cells. The transfection/infection profiles of the samples shown in lanes 1 to 5: 1, nontransfected/not infected; 2, nontransfected/infected; 3, pcDNA3.1/Zeo(−) transfected/infected; 4, C-81 mutant transfected/infected; 5, full-length DCPD transfected/infected. Lanes L and S denote 3-kb linear DHBV DNA and duck serum-derived viral DNA, respectively. (B) 293 cells with stable expression of the DCPD protein. Left, expression of DCPD protein in 293 cells by Western blot analysis; right, viral DNA of bound DHBV particles. Virus binding experiment was carried out at 37°C for 6 h, using a 1:5 dilution of viremic duck serum. For both panels, lanes 1 to 3 represent transiently transfected, nontransfected, and stable transfected 293-4 cells, respectively.