Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jul 30;14:17537. doi: 10.1038/s41598-024-68189-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Study overview illustrating the methods applied to DNA extracted from culture-confirmed M. bovis-positive tissue samples (n = 60) from 57 different African buffalo, selected and included in the study following mycobacterial species identification. After amplifying three target genes (hsp65, rpoB, 16S rRNA), long-read amplicon sequencing was conducted on 56 samples using Oxford Nanopore Technologies (ONT), the Native Barcoding Kit 96 V14, and a single R10.4.1 flow cell. Mycobacterium tuberculosis complex (MTBC) speciation was achieved by amplifying and sequencing the gyrA and gyrB gene targets on 11 randomly selected samples using a single Flongle R10.4.1 flow cell (ONT).