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. 2024 Jul 30;14:17537. doi: 10.1038/s41598-024-68189-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Number of reads with quality score > Q12 obtained using Oxford Nanopore Technologies (ONT) targeted next-generation sequencing (tNGS) across 56 African buffalo tissue samples. Among 78.6% of the samples (n = 44), over 200,000 reads were generated, with a maximum of 897,809 reads. Conversely, the remaining 21.4% (n = 12) of samples yielded reads ranging between 63,705 and 197,343, all of which were included in the analysis. The vertical red dotted line highlights the threshold for the number of reads used for downstream analysis.