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. 2024 Jul 30;14:17537. doi: 10.1038/s41598-024-68189-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Agreement between different genetic targets in detecting and identifying non-tuberculous mycobacteria (NTM) in culture-confirmed Mycobacterium bovis infected African buffalo tissue sample (n = 7). The shorter target PCR (hsp65) demonstrated the highest efficacy in detecting NTM and heterogeneous mycobacterial populations compared to alternative targets. The latter target identified eight NTM in seven samples, rpoB one member of the Mycobacterium avium complex (MAC), and 16S rRNA detected two NTM in distinct samples. In sample KS19028 (bottom two rows), obtained from pooled head lymph nodes, a member of the MAC and an unclassified Mycobacterium sp. were detected in addition to M. bovis. A complete consensus of results was observed across different target PCRs when NTM were detected by two separate genes.