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. 2024 Apr 24;31(8):2433–2446. doi: 10.1007/s43032-024-01567-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Effect of CS and/or TQ applications on apoptotic markers in testicular tissues: A; Casp3 immunoreactivity microphotographs and graph, B; Casp3 mRNA expression, C; TUNEL graph, D; BcL2 immunoreactivity microphotographs and graph, E; TUNEL microphotographs. Levels of apoptotic markers in testicular tissues of control and TQ groups were similar. It was determined that BcL2 immunoreactivity decreased in the CS group compared to the control group, while Casp3 levels and TUNEL-positive apoptotic cells increased. In the CS + TQ group, it was observed that BcL2 immunoreactivity increased compared to the CS group, while Casp3 levels and TUNEL-positive apoptotic cells decreased. *; compared to the control group (p < 0.05), #; Compared to CS group (p < 0.05). A; Casp3 immunohistochemical staining, D; BcL2 immunohistochemical staining, E; TUNEL staining, scale bar; 100 μm. CS; Cisplatin, TQ; Thymoquinone