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Summary of results of tsNIa mutant screens with the yeast two-hybrid system
| Library | NIa sequence mutagenized | No. of mutagenized clones tested | No. of unique tsNIa mutants sequenced | No. of mutations/mutant (mean) | No. of proteinase-active mutants/no. of mutants testedb |
|---|---|---|---|---|---|
| 1a | All | 106 | 19 | 8.4 | 1/19 |
| 2 | Proteinase domain | 2 × 105 | 50 | 3.4 | 2/17 |
Library 1 was composed of two sublibraries.
Proteinase activity was tested by the NIa self-processing assay in E. coli.
