TABLE 2.
Unique suppressor mutant (n = 17) |
---|
NIb-V8A |
NIb-I94T |
NIb-K125E |
NIb-A126T |
NIb-K185E |
NIb-D246G |
NIb-F259Y |
NIb-M322I |
NIb-M322V |
NIb-C380R |
NIb-V444L |
NIb-F479S |
NIb-V85M-M322I |
NIb-C380S-T487S |
NIb-K185E-K421E-F479S |
NIb-A121T-D248G-Y499N |
NIb-K333R-N346H-Y499N |
The entire NIb sequence was mutagenized, and 106 clones were tested. The total number of suppressor mutants isolated was 19, and the mean number of mutations per mutant was 1.4.