Fig. 1. Generation of ANDV mRNA vaccine.

A Schematic representation of the vaccine construct. Expression of Gn/Gc evaluated by flow cytometry in A549 cells at 24 h post transfection with the RNA constructs: mean values from three biologic replicates ± SD, unpaired two-sided T-test (B) and representative primary data (C). D Heatmaps of cytokine expression in A549 and THP1 cells at 24 h after transfection with the U-mRNA and m1Ψ-mRNA from biological triplicates, the scale bar shows log2 fold changes; ND not detected; Source data are provided as a Source Data file. E Electron microscopy of representative VLPs in supernatants of 293T cells transfected with ANDV U-mRNA. Immunostaining with a primary human ANDV antibody cocktail and secondary 6 nm colloidal gold anti-human antibody; the experiment was repeated twice.