Figure 2.

Coculturing with astrocytes enables full maturation of iPSC-MNs. A, A representative micrograph of mouse primary astrocytes. Scale bar, 50 μm. B, Confocal micrographs of primary astrocytes immunoassayed with astrocyte marker, glial fibrillary acidic protein (GFAP), and the nuclear dye Hoechst 33342 (HST). Scale bar, 25 μm. C, Confocal micrograph of iPSC-MNs directly cocultured with astrocytes at 3 weeks postviral infection (wpi). Scale bar, 25 μm. D, Repetitive AP waveforms recorded under current-clamp mode of iPSC-MNs at 3 wpi. E, Degenerated neurons at different time points. Data presented as mean ± SD.