FIG. 2.

Soluble D8L protein bound to BSC40 cell surface CS. Live BSC40 cells were incubated with PBS (black lines) and biotinylated D8L (red lines in panels A through C) or A27L (red lines in panels D through F) protein alone and analyzed with a FACS as described elsewhere (16). Alternatively, biotinylated proteins were mixed with 10, 100, or 1,000 μg of soluble GAGs/ml and analyzed with a FACS, and the cell staining under each condition was shown as a green, blue, or orange line, respectively. The soluble GAGs used in competitions were CS (A and D), DS (B and E), and heparin (C and F).