FIG. 2.
(A) Apoptosis in B19-infected CD36 cells treated with caspase inhibitors. After B19 infection, infected CD36 cells (CD36-I) were cultured in the presence of DEVD-CHO, zVAD-fmk, or IETD-CHO. Uninfected CD36 cells (CD36-NI) were used as a control. At 24, 48, and 72 h, DNA fragmentation was determined by fluorescence microscopy, using the TUNEL assay. (B) NS-1-induced apoptosis in UT7-NS cells treated with caspase inhibitors. UT7-NS cells were induced to express NS-1 by DEX [UT7-NS (DEX)] and cultured in the presence of DEVD-CHO, zVAD-fmk, or IETD-CHO. Noninduced UT7-NS cells (UT7-NS) and UT7-pGRE cells induced by DEX [UT7-pGRE(DEX)] were used as controls. At 24, 48, and 72 h, DNA fragmentation was determined by fluorescence microscopy, using the TUNEL assay.