Skip to main content
. Author manuscript; available in PMC: 2024 Dec 7.
Published in final edited form as: Mol Cell. 2023 Nov 22;83(23):4205–4221.e9. doi: 10.1016/j.molcel.2023.10.037

Figure 2. Fpt1 binds uniquely to RNAPIII transcribed genes and is enriched at regulated tDNAs.

Figure 2.

(A) Genome tracks with pooled ChIP-seq data (n = 3) for TAP-tagged Fpt1, Rpo31, Rpb2 and Rpl13a at the Epi-Decoder locus: tP(UGG)M tRNA gene and YMLWTy1–2 retrotransposon. (B) As in (A), tRNA genes tL(CAA)G1, tK(UUU)K and tT(CGU)K. (C) ChIP-exo metagene profile of Fpt1 (+/− 500 bp from tDNA midpoint) at 261 tRNA genes from a single representative ChIP-exo experiment. Plots of ChIP-exo tag 5’ ends (exonuclease stop sites) are inverted for tags mapping to the non-transcribed (anti-sense) strand. The y-axis shows linear arbitrary units (AU) which are not equal across plotted datasets. Black box at the top: average position of a representative tRNA gene, start and end are indicated with dashed lines. The insert in the lower right corner shows a schematic tRNA gene, containing the internal A- and B-box promoter elements. (D-H) As in (C), ChIP-exo metagene profiles of Rpo31, Brf1, TBP, Tfc4 and Tfc6 (from Rossi et al.58) overlaid with Fpt1. (I) Fpt1 occupancy (ChIP-seq) at housekeeping (n = 30) and regulated (n = 158) tRNA genes. The used classification of tRNA genes is described in Turowski et al.25 and Star Methods. The average Fpt1 occupancy (13142) across 243 tRNA genes is indicated with a dotted horizontal line. Statistics: Welch-corrected unpaired two-tailed t-test.