Figure 3. Fpt1 responds to changing nutrient availability.

(A) Fpt1 enrichment (TAP-ChIP) in glucose, glycerol 2h and 15 min (37°C), and ethanol 2h (n = 4 ± SD). (B) Stacked Fpt1 enrichment from (A). Statistics: Welch-corrected unpaired two-tailed t-test. (C) Fpt1-TAP immunoblot (n = 3) in glucose, glycerol 2h and 15 min (37°C), and ethanol 2h. Pgk1 was used as a loading control. BY4741 is a no-tag control. (D) Quantification of (C), n = 3 ± SD. Statistics: unpaired two-tailed t-test. (E) Fpt1-TAP immunoblot (n = 3) in wild type (WT) and Fpt1 overexpression (OE) in glucose. (F) Fpt1 enrichment (TAP-ChIP) in glucose for WT and Fpt1 OE (n = 3 ± SD). (G) Stacked Fpt1 enrichment from (F). Statistics: Welch-corrected unpaired two-tailed t-test. (H) Left, representative images of Fpt1 localization in live yeast cells in glucose. From top to bottom: Fpt1-GFP (yellow), PP7-NLS-mScarlet-I as a nuclear marker (magenta) and the merged channel. Cellular masks to locate cells and nuclei are indicated with white lines. Scale bar: 3 μm. Right, quantification of Fpt1 nuclear enrichment (n = 3) in glucose (n = 1536 cells), glycerol 2h 37°C (n = 327 cells), and ethanol 2h (n = 771 cells). Circles show data for individual cells; box plots show the distribution of the data where the box indicates the quartiles and whiskers extend to show the distribution, except for outliers. Statistics: bootstrapping (MacKinnon et al.⁶⁷).