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. Author manuscript; available in PMC: 2024 Dec 7.
Published in final edited form as: Mol Cell. 2023 Nov 22;83(23):4205–4221.e9. doi: 10.1016/j.molcel.2023.10.037

Figure 6. Fpt1 occupancy does not depend on active RNAPIII, but requires TFIIIB and TFIIIC.

Figure 6.

(A) Outline of phenanthroline (PH) treatment: cells were grown to mid-log phase and treated with 1,10-phenanthroline (100 μg/mL) or vehicle (0.1% ethanol) for 30 minutes before crosslinking. (B) Rpo31 enrichment (TAP-ChIP) in vehicle and PH-treated cells (n = 3 ± SD). Statistics: unpaired two-tailed t-test. (C-E) As in (B), TAP-ChIP of Brf1, Tfc3, Fpt1 respectively. (F) Schematic of the anchor away (AA) approach. A GFP- and FRB-tagged target protein is depleted from the nucleus after 1 h rapamycin (7.5 μM) treatment. (G) Fpt1 enrichment (TAP-ChIP) upon nuclear depletion of Rpo31 (rapamycin) and DMSO control (n = 3 ± SD). Statistics: unpaired two-tailed t-test. (H-K) As in (G), depletion of Tfc1, Tfc3, Brf1, Bdp1, respectively.