KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-Histone H2B antibody | Abcam Incorporated | RRID: AB_52484 |
| Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) | Abcam Incorporated | RRID: AB_5095 |
| Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed | Abcam Incorporated | RRID: AB_150062 |
| Rabbit Anti-Mouse IgG H&L (HRP) | Abcam Incorporated | RRID: AB_6728 |
| Chemicals, peptides, and recombinant proteins | ||
| Fetal Bovine Serum, dialyzed | Gibco | A3382001 |
| TrypLE™ Express Enzyme (1X), no phenol red | Gibco | 12604013 |
| Penicillin-Streptomycin | Gibco | 15070-063 |
| Fibronectin bovine plasma | Sigma-Aldrich | F4759 |
| mTeSR™1 | STEMCELL Technologies | 85850 |
| HEPES | Gibco | 15-630-080 |
| Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free | Corning | 356231 |
| DMEM/F-12, HEPES, no phenol red | Gibco | 11039021 |
| StemPro™ Accutase™ Cell Dissociation Reagent | Thermo Fisher | A1110501 |
| KnockOut™ Serum Replacement | Thermo Fisher | 10828010 |
| ROCK inhibitor | STEMCELL Technologies | Y-27632 |
| Hoechst | Thermo Fisher | 62249 |
| DPBS, without Ca++ or Mg++ | Gibco | 14190-144 |
| PBS | Corning | 21-040-CV |
| N-Hydroxysuccinimide | Sigma-Aldrich | 130672 |
| EDC | Sigma-Aldrich | 341006 |
| Bovine Serum Albumin | Sigma-Aldrich | A9647 |
| Triton™ X-100 | Sigma-Aldrich | A9647 |
| Paraformaldehyde | Santa Cruz | sc-281692 |
| Normal donkey serum | Jackson ImmunoResearch Laboratories Inc.) | 017-000-121 |
| Formamide | Thermo Fisher | AM9344 |
| 2-deoxy-D-glucose | Sigma-Aldrich | D8375 |
| Antimycin A | Sigma-Aldrich | A8674 |
| D-glucose | Thermo Fisher | A2494001 |
| 40% acrylamide | BIO-RAD | 1610140 |
| 2% Bis Solution | BIO-RAD | 1610142 |
| (3-Aminopropyl)trimethoxysilane | Sigma-Aldrich | 281778 |
| Glutaraldehyde solution | Sigma-Aldrich | G6257 |
| Ammonium Persulfate | BIO-RAD | 1610700 |
| TEMED | BIO-RAD | 1610801 |
| Sulfo-SANPAH (sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino)hexanoate) | Thermo Fisher | 22589 |
| Trypan Blue solution | Sigma-Aldrich | T8154 |
| Experimental models: Cell lines | ||
| Human induced pluripotent stem cells (hiPSCs) | Coriell Institute | AICS-0096-074 |
| Software and algorithms | ||
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| MATLAB | The MathWorks, Inc. | https://www.mathworks.com/products |
| VistaVision | ISS | https://iss.com/software/vistavision |
| Other | ||
| Inverted microscope | Leica | DMIRE2 |
| Olympus confocal scanning system | ISS | Alba |
| DriveAFM | Nanosurf Inc. | https://www.nanosurf.com/en/products |
| Transmission electron microscope | JEOL | JEOL JEM 1010 TEM |
| Scanning Electron Microscope | Hitachi | Hitachi S-4800 FE-SEM |
| FemtoJet 4i and InjectMan 4 | Eppendorf | https://www.eppendorf.com/product |