Figure 3.

Effect of FGF2 priming on the expression levels of catalase and glutathione peroxidase 1/2 (GPx1/2) in individual donor-derived dental pulp cells (DPCs) after treatment with H₂O₂. Each DPC clone was treated with 0.3 mM H₂O₂. At 0 h (A, B) or 3 h (C) after the treatment, the expression levels of the catalase (A, C) and GPx1/2 (B, C) proteins were determined by Western blotting, together with the evaluation of β-actin as a loading control. The results obtained for catalase, or GPx1/2, are shown as the mean ± standard deviation. Significant differences from the FGF2 non-treated groups were determined using two-way ANOVA with post-hoc Tukey’s multiple comparison test. *P < 0.05; **P < 0.01, n = 4–5 for each group. Correlation analyses of the expression levels of catalase (D) or GPx1/2 (E) with cell viabilities at 24 h after treatment with 0.3 mM H₂O₂.