Figure 6.

Effect of RTA402 on the expression levels of the heme oxygenase-1 (HO-1) and NAD(P)H-quinone dehydrogenase 1 (NQO1) proteins in DP31 and DP296 primed with basic fibroblast growth factor (FGF2). After priming with/without FGF2, each dental pulp cell (DPC) clone was treated with various concentrations of RTA402 (0, 50, and 150 nM) for 24 h. (A) The expression of the HO-1 and NQO1 proteins was determined by Western blotting, together with the evaluation of β-actin as a loading control. The results obtained for HO-1 (B) or NQO1 (C) are shown as the mean ± standard deviation. Significant differences between the two groups were determined by two-way ANOVA with post-hoc Tukey’s multiple comparison test. *P < 0.05; **P < 0.01, and ***P < 0.001 vs DPC-S untreated with H₂O₂ and ^#P < 0.05; ^##P < 0.01, and ^###P < 0.001 vs DPC-F untreated with H₂O₂, n = 4 for each group.